mouse brain cdna mate (TaKaRa)
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mouse brain cdna mate
Mouse Brain Cdna Mate, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+brain+cdna/Mouse+Brain+QUICK-Clone+cDNA/pm40812511-49-21-28
Average 93 stars, based on 524 article reviews
Mouse Brain Cdna Mate, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+brain+cdna/Mouse+Brain+QUICK-Clone+cDNA/pm40812511-49-21-28
Average 93 stars, based on 524 article reviews
mouse brain cdna mate - by Bioz Stars,
2026-09
93/100 stars
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Amplification:Article Title: Weak and tunable adhesion-clutch drives rapid cell migration and glioblastoma motility Article Snippet: Unphosphorylated (S101A/S249A: AA) and phosphomimic (S101D/S249D: DD) mutants of rat shootin1b were generated with the QuickChange II site -directed mutagenesis kit (Stratagene) to replace each serine (S) with alanine (A) or aspartate (D) using the following primers : S101A Fw (5’-AAAAGAATCGCCATGCTATACATG-3’), S101A Rv (5’-CATGTATAGCATGGCGATTCTTTT-3’), S101D Fw (5’-AAAAGAATCGACATGCTATACATG-3’), S101D Rv (5’-CATGTATAGCATGTCGATTCTTTT-3’), S249A Fw (5’-AAGAGACAAGCCCACCTTCTGCTG-3’), S249A Rv (5’-CAGCAGAAGGTGGGCTTGTCTCTT-3’), S249D Fw (5’-AAGAGACAAGACCACCTTCTGCTG-3’), S249D Rv (5’-CAGCAGAAGGTGGTCTTGTCTCTT-3’). cDNA of shootin1b DD or AA mutants was sub-cloned into pCMV-flag vector . .. To generate AcGFP-integrin β1 and β2 intracellular domains (ICDs), integrin β1- or β2-ICD was amplified Article Title: Glaucoma-associated Optineurin mutations increase transmitophagy in a vertebrate optic nerve Article Snippet: All the OPTN mutations (E50K, M98K, F178A, D474N, E478G, and H486R) were then introduced into this construct by QuikChange® Site-Directed Mutagenesis Kit (Stratagene) using the corresponding primers (Primer List below, mutagenized bases are shown in uppercase) and verified by Sanger sequencing. .. The final step of placing all these under control of the full 20kb of the zebrafish Isl2b promoter, which drives RGC-specific expression in both zebrafish ( ) and X. laevis , was accomplished by recombineering (Chan et al., 2007), carried out with minor modifications ( ). pCS2(Isl2b:Tom20-mCherry-Apex2_msSOD2UTR): First, to improve the mitochondria targeting efficiency of the transmitophagy reporter, pCS2(1kb Isl2b):Mito-EGFP-mCherry , the SV40pA was replaced by the 3’UTR of msSOD2 ( ) amplified from Polymerase Chain Reaction:Article Title: Weak and tunable adhesion-clutch drives rapid cell migration and glioblastoma motility Article Snippet: Unphosphorylated (S101A/S249A: AA) and phosphomimic (S101D/S249D: DD) mutants of rat shootin1b were generated with the QuickChange II site -directed mutagenesis kit (Stratagene) to replace each serine (S) with alanine (A) or aspartate (D) using the following primers : S101A Fw (5’-AAAAGAATCGCCATGCTATACATG-3’), S101A Rv (5’-CATGTATAGCATGGCGATTCTTTT-3’), S101D Fw (5’-AAAAGAATCGACATGCTATACATG-3’), S101D Rv (5’-CATGTATAGCATGTCGATTCTTTT-3’), S249A Fw (5’-AAGAGACAAGCCCACCTTCTGCTG-3’), S249A Rv (5’-CAGCAGAAGGTGGGCTTGTCTCTT-3’), S249D Fw (5’-AAGAGACAAGACCACCTTCTGCTG-3’), S249D Rv (5’-CAGCAGAAGGTGGTCTTGTCTCTT-3’). cDNA of shootin1b DD or AA mutants was sub-cloned into pCMV-flag vector . .. To generate AcGFP-integrin β1 and β2 intracellular domains (ICDs), integrin β1- or β2-ICD was amplified Article Title: Functional Diversity of Human Basic Helix-Loop-Helix Transcription Factor TCF4 Isoforms Generated by Alternative 5′ Exon Usage and Splicing Article Snippet: .. Full-length coding regions of NeuroD2 and Id2 were PCR-amplified from Article Title: Leucine-Rich Repeats and Transmembrane Domain 2 Controls Protein Sorting in the Striatal Projection System and Its Deficiency Causes Disturbances in Motor Responses and Monoamine Dynamics Article Snippet: .. Mouse Lrtm2, Gad1, and Gabbr1 cDNAs were PCR-cloned from Control:Article Title: Nogo receptor is involved in the adhesion of dendritic cells to myelin Article Snippet: Assays used for mouse mRNA detection were as follows: NgR1 (Mm00452228_m1), NgR2 (Mm01336368_g1), LINGO-1 (Mm01173306_m1), TROY (Mm00443506_m1), and p75 NTR (Mm00446296_m1). .. 18 s rRNA was measured in each sample as an endogenous control in order to control for varying cDNA concentrations and human or Article Title: Glaucoma-associated Optineurin mutations increase transmitophagy in a vertebrate optic nerve Article Snippet: All the OPTN mutations (E50K, M98K, F178A, D474N, E478G, and H486R) were then introduced into this construct by QuikChange® Site-Directed Mutagenesis Kit (Stratagene) using the corresponding primers (Primer List below, mutagenized bases are shown in uppercase) and verified by Sanger sequencing. .. The final step of placing all these under control of the full 20kb of the zebrafish Isl2b promoter, which drives RGC-specific expression in both zebrafish ( ) and X. laevis , was accomplished by recombineering (Chan et al., 2007), carried out with minor modifications ( ). pCS2(Isl2b:Tom20-mCherry-Apex2_msSOD2UTR): First, to improve the mitochondria targeting efficiency of the transmitophagy reporter, pCS2(1kb Isl2b):Mito-EGFP-mCherry , the SV40pA was replaced by the 3’UTR of msSOD2 ( ) amplified from Positive Control:Article Title: Nogo receptor is involved in the adhesion of dendritic cells to myelin Article Snippet: Assays used for mouse mRNA detection were as follows: NgR1 (Mm00452228_m1), NgR2 (Mm01336368_g1), LINGO-1 (Mm01173306_m1), TROY (Mm00443506_m1), and p75 NTR (Mm00446296_m1). .. 18 s rRNA was measured in each sample as an endogenous control in order to control for varying cDNA concentrations and human or Sequencing:Article Title: Functional Diversity of Human Basic Helix-Loop-Helix Transcription Factor TCF4 Isoforms Generated by Alternative 5′ Exon Usage and Splicing Article Snippet: .. Full-length coding regions of NeuroD2 and Id2 were PCR-amplified from Expressing:Article Title: Glaucoma-associated Optineurin mutations increase transmitophagy in a vertebrate optic nerve Article Snippet: All the OPTN mutations (E50K, M98K, F178A, D474N, E478G, and H486R) were then introduced into this construct by QuikChange® Site-Directed Mutagenesis Kit (Stratagene) using the corresponding primers (Primer List below, mutagenized bases are shown in uppercase) and verified by Sanger sequencing. .. The final step of placing all these under control of the full 20kb of the zebrafish Isl2b promoter, which drives RGC-specific expression in both zebrafish ( ) and X. laevis , was accomplished by recombineering (Chan et al., 2007), carried out with minor modifications ( ). pCS2(Isl2b:Tom20-mCherry-Apex2_msSOD2UTR): First, to improve the mitochondria targeting efficiency of the transmitophagy reporter, pCS2(1kb Isl2b):Mito-EGFP-mCherry , the SV40pA was replaced by the 3’UTR of msSOD2 ( ) amplified from Plasmid Preparation:Article Title: Experimental and computational evidence that Calpain-10 binds to the carboxy terminus of Na V 1.2 and Na V 1.6 Article Snippet: .. The procedure followed for Y2H screens was described before by Vega et al. . For the initial screen, yeast cells of strain L40 were co-transfected with the bait plasmid pSN12CT together with a commercial plasmid library derived from adult Derivative Assay:Article Title: Experimental and computational evidence that Calpain-10 binds to the carboxy terminus of Na V 1.2 and Na V 1.6 Article Snippet: .. The procedure followed for Y2H screens was described before by Vega et al. . For the initial screen, yeast cells of strain L40 were co-transfected with the bait plasmid pSN12CT together with a commercial plasmid library derived from adult Activation Assay:Article Title: Experimental and computational evidence that Calpain-10 binds to the carboxy terminus of Na V 1.2 and Na V 1.6 Article Snippet: .. The procedure followed for Y2H screens was described before by Vega et al. . For the initial screen, yeast cells of strain L40 were co-transfected with the bait plasmid pSN12CT together with a commercial plasmid library derived from adult |
